The Study of immobilization technology on a Surface Acoustic Wave Devices Biosensor
| Autor: | 張佑銘 |
|---|---|
| Rok vydání: | 2004 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 92 Surface Acoustic Wave (SAW) biosensor system has high specificity, high sensitivity and also has a real time analysis response. In this system, any mass change occurred at the piezoelectric surface after an antibody-antigen reaction is reflected either by an increase or decrease in the frequency of the piezoelectric crystal. Surface Acoustic Wave (SAW) biosensor system could be used in different clinical sample, including bacteria, virus, immunoglobulin and DNA etc. It could be proved that by clinical examination Surface Acoustic Wave (SAW) biosensor possess greater potential in the future. The purpose of this study is to find methods to immobilization protein on the Aluminum Nitride (AlN) piezoelectric surface. In this research, different physical and chemical protein immobilization methods were studied and compared with respect to their performance as well as the processes involved in the preparation. Among chemical methods, the use of 11-mercaptoundecanol for gold electrode surface activation followed by carbodiimide condensation presents the best responses when A Type Blood Cell, used as insolubilized material for A Type Blood Cell and Anti-A Type Blood Cell diction. The experimental results show that a novel method of immobilizing antibodies on Aluminum Nitride (AlN) film for Surface Acoustic Wave (SAW) was successfully developed by using amino plasma treatment. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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