The Activity of Trypsin upon Complexation with Thiolalkylated Crown Ether Modified Gold Nanoparticles

Autor:	I-Fang Li, 李宜芳
Rok vydání:	2004
Druh dokumentu:	學位論文 ; thesis
Popis:	92 Two crown ether, 2-[(10-Mercaptodecyl)oxy]methyl-18-crown-6 (18-Crown-6) and 2-[(10-Mercaptodecyl)oxy]methyl-12-crown-4 (12-Crown-4), functionalized gold nanoparticles have been characterized both in its TEM micrograph and its effect on trypsin trytic activity. In TEM micrographic studies, Au/18-crown-6 conjugates organized into a uniform 2D monolayer with close hexagonal packing, while Au/12-crown-4 exhibited both 2D and 3D assembles, in the potassium phosphate buffer (10 mM, pH 8). The trypsin tryptic activity was studied as a function of Au/Crown concentrations (1 nM, 5 nM, 10 nM). It was found that the initial rate in the 1 nM of Au/Crown showed about 40% decrease compared with pure enzyme; however, with the increase of Au/Crown concentration, the activity increased.
Databáze:	Networked Digital Library of Theses & Dissertations
Externí odkaz:	http://ndltd.ncl.edu.tw/handle/y5spec Zobrazit plný text záznamu