Study on the n-type hydrogenated amorphous silicon extended gate ion-sensitive field effect transistor for measuring the berberine of the alkaloid.
| Autor: | Shin-Cheng Chang, 張欣城 |
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| Rok vydání: | 2003 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 91 Berberine (BE) is an alkaoid which is derived from berberis aristata. It is a antiseptic medicine which has inhibitive effects for the streptococcus, staphylococcus and shigella and is widely used as a stomach and bowel medicine. BE is an anti-inflammatory active ingredient for heart and intestinal disorders, which is recently reported to prossess anti-tumor promoting activities and anti-lipase effects. However, for the determination of berberine suffer from some drawbacks as being time consuming with a cumbersome sample pretreatment or requiring sophisticated instrumentation. Icomparison with other types of measuring the drug, the ion sensitive field effect transistor (ISFET) has certain well-known advantages: miniaturization, solid structure, high sensitivity, low cost and multi-detection potential. If fabricate a good response of the berberine drug sensitive field effect transistor (DrugFET) successfully, and that will improve the manner of measuring berberine. Which has great influence to promote the development of the medical treatment. In this study, we employ the advantage of the extended gate field effect transistor (EGFET) to fabricate a a kind of drug sensitive field effect transistor (DrugFET) sensor for berberine chloridum with silicotungstic acid as active material. The EGFET membrane of the N-type a-Si:H and SnO2/ITO was be used in the fabrication of the element. This DrugFET is prepared by the combination of the EGFET and a drug sensitive member, which is composed of active material silicotungstic acid, plasticizer dioctyl phthalate, PVC power and tetrahydrofuran solvent. The separative structure of the berberine DrugFET has a linear range from 1.0×10-3 ~5.0×10-6mol/L with a solpe of 50.62 mV/decade(25℃). The advance of the EGFET are light insensitivity, simple to passivate and packages, the flexibility of shape of the extended gate area, etc. This structure is suitable for application for a disposable biosensors and Drug-sensors. When compare the measurement manner of the berberine DrugFET and the other manner. The extended gate berberine silicotungstic-acid DrugFET is more suitable to be a DrugFET in measuring the berberine. The DrugFET device will measure the fundamental sensitivity when it was fabricated. And also measure the stability, reproduction, selectivity. The influence of the pH will be understood in the berberine solution by the adding the acid of the HCl and base of the NaOH. And the interference experiment was used the NaCl for interference research. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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