The Comparison of Tissue Culture System of Three Species of Brassicaceae Plant
| Autor: | Pei-Ling Shen, 沈佩伶 |
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| Rok vydání: | 2003 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 91 This study was intended to use various part of three species of Brassicaceae as the receptacle to induce the callus and adventitious bud regeneration.To reach the purpose of this study, we composited MS medium with various concentration of plant growth regulators to determine the efficiency of callus induction and bud regeneration. Results as follows: 1.The petiole of Arabidopsis thaliana as the receptacle, can be induced callus formation by MS medium added with NAA 0.5 ppm & BA 1 ppm, then transplant the callus to MS medium added with iPA 1 ppm & IAA 0.1 /0.2 ppm to induce root and shoot formation. A complete plantlet will be observed after 45days. 2. The cotyledon of turnip as the receptacle, can be induced callus formation by MS medium added with kinetin 1 ppm.100 % receptacles form callus after 7 days. 3. The petiole of cabbage as the receptacle, can be induced callus formation by MS medium added with NAA 5 ppm & Kinetin 0.5 ppm.90% receptacles form callus after 7 days. In this experiment, A plantlet can be observed in Arabidopsis thaliana. The turnip and cabbage can only develop to the callus formation and with the bud formation accidentally, but it need to work hard in the aspects of plant cultivate. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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