Microgravity Inhibit Growth and Differentiation of Neural Progenitor Cells
| Autor: | lee li-jung, 李麗蓉 |
|---|---|
| Rok vydání: | 2003 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 91 Introduction：During the travel in space, the cells of human body could sense the change of gravity in environment and develop a series of physiological adaptation. In experimental microgravity condition, many cells could generate obviously physiological changes in cell growth, differentiation, and in response to stress. Recent years, neural stem cells have been cultured in experimental condition, which possess the ability as in embryo stage to proliferate, self-renew, and differentiate to all kinds of cells in nervous system that could be used in treating many neurodegenerative diseases in the future. Purpose：To investigate these effection of cell growth, proliferation and differentiation in experimental microgravity condition. Methods： In this study, we have successfully cultured neural progenitor cells from cerebral cortex and retina of Sprague-Dawley new born. Using the rotary cell culture system, Results：1. We found that microgravity could slow the growth curve in comparison to those of normal gravity. 2. Microgravity could decrease the accumulated nitric oxide in comparison to those of normal gravity. 3. With MTT assay, we found that microgravity could decrease the cell viability. 4. With TUNEL stain, we also found that microgravity could induce DNA fragmentation and apoptosis of the neural progenitor cells, which could be block by caspase inhibitor. 5. Using immunocytochemical assay, the ratio and rate of neuronal differentiation could also be diminished by microgravity in these cells. Conclusion： In our study, microgravity could inhibit the growth and the differentiation of neural progenitor cells, which could be due to enhancement of nitric oxide secretion that induced cytotoxicity, activation of caspase and finally apoptosis. Further studies are needed to analyze the detail mechanisms in apoptosis induced by microgravity, which will be helpful in understanding the adaptation in human exploring the universe in the future. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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