Vascular smooth muscle cells hypertrophy mediates
| Autor: | CHUNG CHI HSU, 徐中濟 |
|---|---|
| Rok vydání: | 2003 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 91 The endothelin-1(ET-1) is a peptide of 21 amino acid with potent biological activities. ET-1 is associated with aortic vascular smooth muscle cells (VSMCs) hypertrophy in humans and in hypertensive animal models. Hypertrophy would refer to increase in smooth muscle cell size and DNA content, and it would also refer to increase in total protein content. The pathogenesis of vascular hypertrophy is a complex interaction between ET-1 and its receptors-ETA and ETB. Therefore the purpose of this study was to investigate the relationship between VSMCs hypertrophy and endothelin receptor expression in cultured VSMCs cell line A-10, and primary culture VSMCs from SHR and WKY rats. In culture system, we found that all groups of VSMCs would hypertrophy in confluence condition；the expression of ETA receptor would increase but the expression of ETB would decrease. BQ-123, the antagonist of ETA receptor, would inhibit hypertrophy, BQ-788 the antagonist of ETB receptor, would not. In confluence, linolenic acid not only inhibited hypertrophy but also influenced the expression of ETA and ETB receptor, it’s the same as in subconfluence. Thus, we concluded that VSMCs hypertrophy was related to the expression of ET-1 and its receptors of ETA and ETB and the formation of gap junction. All these factors may be critical to the elevate of blood pressure and be relevant to the pathogenesis of hypertension. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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