Use of response surface methodology for the production of mycelium of Cordyceps sinensis in a 4-L fermentor
| Autor: | Jen-Ping Huang, 黃任平 |
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| Rok vydání: | 2002 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 90 Dongchongxiachao is a traditional precious Chinese medicine, chiefly composed of Cordyceps sinensis parasiting on larva of Hepialus spp. It is effective against tumors and is also capable of enhancing one’s immune system. Recently, since Dongchongxiachao’s production rate is going downhill, alternative materials have to be found to replace it. Cordyceps sinensis mycelium production by fermentation has as good medical effect as that of the natural one. Besides, it can be produced in large quantities in a short time. Therefore, production of Dongchongxiachao should be of great potential in the future. This study utilize response surface methodology to investigate the optimal growth condition for Cordyceps sinensis in a 4-L fermentor. Shake flask experiment was performed first on a rotary shaker. Effect of carbon and nitrogen sources on mycelium production was investigated. Medium with optimal carbon and nitrogen combination was then used in the experimental design of response surface methodology of having two factors and two levels. The result of optimization of response surface methodology as then applied from shake flask to fermentor experiments. The factors investigated were mixing rate and temperature. This experiment also was based on experimented design of response surface methodology with two factors and two levels. Results of these experiments reveal that the best carbon source is found to be sucrose whereas soybean powder serves as best nitrogen source. In shake flask experiments, using the central composite design with two factors and two levels involving sucrose and soybean powder, the results showed that the optimum sucrose and soybean powder concentrations are 4.53％ and 2.67％, respectively. These results were obtained after 144 hours cultivation and after regressional analysis. The maximum yield of mycelium was 33.2 mg/mL with an average of 32.4 mg/mL. In fermentor experiments, using the central composite design with two factors and two levels involving rotation rate and temperature, the results showed that the optimum rotation rate and temperature are 216rpm and 20.75℃, respectively. These results were obtained after 96 hours cultivation and after regressional analysis. The maximum yield of mycelium was 37.4 mg/mL with an average of 37.3 mg/mL. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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