Relationship between Na+, K+ -ATPase and carbonic anhydrase and salinity changes in the widely distributed fiddler crab Uca lactea
| Autor: | 李彥志 |
|---|---|
| Rok vydání: | 2001 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 90 Uca lactea is a semi-terrestrial crab and widely distributes along the west coast of Taiwan. It lives in intertidal areas and is subjected to large daily and seasonal changes in salinity. Previous literature indicated that not only Na+, K+-ATPase but also carbonic anhydrase (CA) are important in osmoregulation. However, these two enzymes have never been studied simultaneously for the osmoregulation of brachyuran crabs. From previous studies, the CA activity in different pairs of gills was species-specific. No significant difference among gill CA activity was found in one crab species with high salinity treatment while a significantly higher CA activity in the posterior gills in a Uca species was reported in another report. In my study, I examined the variation of these two enzymes in Uca lactea. The experiment started with salinity acclimation of 5, 25, 45 ‰ and osmolality, Na+, K+-ATPase and CA activity and western blotting were examined. From osmolality results, Uca lactea is a hyper-hypo osmoregulator. Both Na+, K+-ATPase and CA activity were higher in diluted seawater and in posterior gills. The results from western blotting had a similar pattern in that Na+, K+-ATPase and CA were higher in posterior gills. The CA activity in Uca lactea was higher in 45 ‰ than in 25 ‰, suggesting that the CA may be more important than Na+, K+-ATPase in osmoregulation in concentrated seawater. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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