Preparation and application of morphine Immunosensors
| Autor: | Hung-wai Chang, 張宏維 |
|---|---|
| Rok vydání: | 2002 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 90 Current technology for determination of morphine in blood or urine is often to use the chromatographic methods such as GC and HPLC, but most of these methods are time consuming. To solve this problem, a morphine optical immuno-sensor and a quartz piezoelectric crystal sensor based on anti-morphine antibody and Glucose-6-phosphate dehydrogenase labeled with morphine (G6PDH-morphine) for opiate solutions were designed and prepared in this study. The optical morphine sensor exhibited good sensitivity with a detection limit of 113 ng/mL which was far below the allowed concentration (300 ng/mL) in law for morphine in biological solutions. The optical sensor also showed a high reproducibility with a relative standard deviation (RSD) of approximately 3 %. The interference of major biological species in simulated serum and urine solutions for the determination of morphine with the optical immuno-sensor was investigated and discussed. In the preparation of the quartz piezoelectric sensor, immobilized fullerene C60 - G6PDH-morphine was synthesized and applied as a coating material on quartz piezoelectric crystals for the immunoassay of anti-morphine antibody in aqueous solutions. The oscillating frequency of the G6PDH-morphine coated-piezoelectric crystal of the piezoelectric sensor decreased by adsorption of anti-morphine antibody in solutions. The piezoelectric crystal sensor showed nearly no interference from various biological species in simulated serum. The piezoelectric crystal sensor based on immobilized C60-G6PDH-morphine for anti-morphine antibody also exhibited good sensitivity and good reproducibility. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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