In vitro self-assembly of S-layer proteins extracted from Deinococcus radiodurans cell wall: size analysis of the assemblies
| Autor: | Hung-Sheng Lin, 林宏昇 |
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| Rok vydání: | 2002 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 90 We have demonstrated the in vitro self-assembly capacity of Deinococcus radiodurans IR S-layer proteins (SLPs) by using a protocol involving removing of a hydrogen-bond-breaking agent that had added in a cell wall preparation to extract protein molecules of interest. The resulted self-assemblies (SAs) were micron-scale sheet structures when observed under light and electronic microscope. Also observed was the regular pattern on SAs’ surface (TEM) and multilayer structures (field-emission SEM). Furthermore, we determined the SAs’ particle size distribution (COULTER® Multisizer II) in the course of SAs formation. These results suggested that the self-assembly was time- and temperature-dependent and was mainly due to ~100 kD cell-wall proteins (SDS-PAGE). Coulter size analysis could be of potential application in the study of mechanism of self-assembly in particular and in the field of nanotechnology in general. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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