利用固定化菌體生產酒精之研究
| Autor: | Chiu Wen Chuan, 邱文娟 |
|---|---|
| Rok vydání: | 2002 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 90 Ethanol can be used in wide application, like fuel, co-surfanctant and solvent. With increasing the care of environment, the amount of ethanol is growing up. Recently, the main method of ethanol production is fermentation using microorganisms. In this paper, we used the isolated cell from the ferment for brewing to discuss the optimal production conditions for free cell and immobilized cell with alginate in batch experiments. However, we also observed the operation stability in repeated batch experiments with immobilized cells, including ALG and APU. The optimal temperature and pH for are 30℃ and 4.5. When the optimal glucose concentration is 250 gl-1, the ethanol productivity is 0.61 g g-1 cell h-1. Its ethanol tolerance is 8 % (w/v). After immobilization, the optimal pH of ALG is shift and increased to 5.0, but the optimal temperature is still 30℃. When the glucose concentration is 300 gl-1, ALG had the best ethanol productivity. However, the ethanol tolerance can be increased to 12 % (w/v). In the optimal conditions for immobilized cells (ALG), we used ALG and APU for repeated batch experiments. As data shows the relative fermentation capacities and production yield increased with repeated times increased. Due to the mechanical strength of ALG is lower, after fourth time batch experiment most of all are broken and can not be used. After six times of batch experiments, the relative fermentation capacity of APU is still above 100 %. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|