Using antisense oligonucleotides to study the role of protein kinase C beta-1 in the rat liver cancer cell line GP7TB
| Autor: | Lin Bih-Shiang, 林碧香 |
|---|---|
| Rok vydání: | 2001 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 89 Protein kinase C beta-1 (PKC-β1) is one of conventional protein kinase C. The influence of PKC-β1 on secretion, gene expression and cell proliferation is varied in different cell type. There are studies showed that over-expression of the PKC-β1 can stimulate the proliferation and oncogenesis of fibroblast. While other investigations found that cell growth and tumor development were inhibited when over-expressing PKC-β1 in colon cancer cells. Because of the opposite role of PKC-β1 in cell proliferation and differentiation, we are interested in studying the influence of decreasing PKC-β1in rat liver cancer cell line GP7TB. In this thesis, we used antisense oligonucleotide to inhibit the expression of PKC-β1 in GP7TB cells. After the suppression of PKC-β1, cell cycle in S phase was prolonged. An increase in the level of cyclin D1 and a decrease in cell cycle-regulatory molecules, p27, the CDK inhibitor, were found might promote the cell entering S phase. Upon the decreasing of PKC-β1, increases in anchorage independent growth and expression of stem cell marker, c-kit, hepatocyte growth factor and it’s receptor, c-met were also found These results suggest that, in GP7TB cells, depletion of PKC-β1 by antisense strategies, the cell cycle in S phase was prolonged and the tumorigenicity of the cell was increased. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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