Apoptosis of Vascular Cells and Dysfunction of Aorta in Cecal Ligation and Puncture-induced Sepsis in Rats
| Autor: | Chou, Yeh-Cheng, 周曄竫 |
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| Rok vydání: | 2001 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 89 Sepsis exhibits a complicated pathogenesis where serves sepsis with hypotention and hypoperfusion can lead to multiple organ failure. Apoptosis (programmed cell death) in both immune and parenchymal cells during sepsis, also occurs. We thus asked whether cell apoptosis of blood vessel concurred with the vascular dysfunction during sepsis. Adult male WKY were used and sepsis was induced by cecal ligation and puncture (CLP). We found that in CLP rats (10h and 20h), blood pressure, glucose level and serum pH decreased; heart rate and lactate level increased; both TNF-a and IL-1b level also elevated. Aortic rings and segments were isolated for contractile response and apoptosis study, respectively. Following agonist-induced contraction, ACh-induced relaxation, SNP-induced relaxation (with L-NNA), and endothelial suppression (with and without L-NNA) were found to decrease in CLP rats suggesting that both endothelial-dependent and -independent relaxation as well as basal endothelial NO were reduced. TUNEL analysis revealed significant apoptosis of vascular cells (~13%) and confirmed by DNA ladder pattern. These results indicate that apoptosis of vascular cells and vascular dysfunction were observed in the same blood vessel during sepsis and could suggest association between them. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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