Analysis of Mouse Interleukin-15 Receptor Alpha Chain Promoter
| Autor: | Yaw-Wen Hsu, 許耀文 |
|---|---|
| Rok vydání: | 2000 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 88 Interleukin-15 ( IL-15 ) is a T cell growth factor identified in 1994. In spite of lacking sequence homology between IL-15 and IL-2, both cytokine are members of the four-helix bundle cytokine family, which supports lymphocyte growth. Differences in function of IL-15 and IL-2 have also been found. In contrast to IL-2, IL-15 drives the differentiation of thymic progenitors toward NK and gd T cells. IL-15 is also a survival factor for resting and activated CD8aa+ intestinal intra epithelial lymphocyte. These observations suggest that IL-15 and IL-2 use different mechanisms to affect cell physiology. The receptor of IL-15 and IL-2 consist of three subunits, including the shared b and g subunits and the unique a subunit. Previous studies on IL-2R clearly demonstrated that b and g subunits mediate signal transduction. The different function between IL-15 and IL-2 may result from the different receptor a subunits. IL-2 receptor a subunit has a very short intracytoplasmic tail, which can not transduces signal. Whereas the IL-15Ra has signal transduction function although its intracellular domain is only 37 a.a. long. In this study, I analyzed the promoter activity of a 2.41kb fragment immediately 5’ of ATG of the IL-15Ra gene. Using luciferase reporter system in BaF3 cells, the most 3’ 0.4kb fragment is the candidate promoter region, while the next 5’ 0.7kb fragment may contain repressor, and the 5’ 1.1kb before 0.7kb may relate to IL-15Ra expression at late stage. The information generated from these studies lead to future study in the control of IL-15Ra expression. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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