A Study on the Population Genetic Variation of Buxus liukiuensis by the Method of RAPDs
| Autor: | MEI-YI HUANG, 黃美玉 |
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| Rok vydání: | 2000 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 88 Buxus liukiuensis, a rare plant of Taiwan and Lyukyus, usually occurs at eastern and southern parts of Taiwan, and its elevations ranges form 100 to 1,500 meters above sea level, at limestone sites:they occupy a small isolated patches mixed with other trees in forests. This aim of this study is to research the genetic variation of Buxus liukiuensis Makino. by RAPDs analysis : a total of 150 samples of Buxus liukiuensis which are representing the populations of eight area were collected.A test of random amplified polymorphic DNA to identify its genetic variation .and Amplification with 9 primers to yield a total of 97 polymorphic RAPDs bands were recorded.Among them, 92 bands are polymorphism(94.85﹪),and 8 DNA bands are monomophism(8.25﹪). A UPGMA cluster analysis was performed on the Dice''s similarity matrix which derives form eight populations of plants by Ntsys. Partition the genetic variation into within and between populations of Buxus liukiuensis through POPGENE analysis indicated 36.54﹪of variation is between populations while63.46﹪of variation within population.It is suggested,based on the studies,there is not or lower gene interflow existed among populations,indicating great genetic differentiation which was mostly caused by geographical isolation occurred. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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