Cryopreservation of Bovine Oocytes
| Autor: | Tzu-Kuan Kuo, 郭子寬 |
|---|---|
| Rok vydání: | 2000 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 88 Experiments were conducted in an effort to develop a more effective open pulled straw (OPS) vitrification procedure for bovine oocytes. Different parameters likely to influence the survival of bovine oocytes after a vitrification procedure were evaluated：straw diameter, oocyte meiotic stage, exposed time in vitrification solution, sucrose concentration and combination of cryoprotectants. A reduction in the straw diameter from 1.7 mm to 0.4 mm increased the maturation rate from 37％ to 54.3％. Oocytes were cryopreserved at different maturation stages（0, 6, 12 and 18 hr）showed a higher maturation rate at 6 hr and 12 hr（58.5％ and 57.6％, respectively）than 0 hr and 18 hr（30.6％ and 33.2％, respectively）. To access EG-DMSO toxicity, the oocytes were equilibrated in EG and DMSO both at a concentration of 10% for 1, 5 and 10 min. The reduction maturation rates were showed（57.1％, 41.1％ and 34.0％, respectively）when exposed time were increased. When bovine oocytes were exposed to different concentrations of cryoprotectants, the maturation rates were the highest with EG-DMSO group, than followed by EG-PG and PG-DMSO groups. In summary, oocytes were maturated in vitro for 6 hr, 0.4 mm straw diameter, 10％ EG-10％ DMSO for 1 min exposed time and 0.3 M sucrose with cryoprotectant resulted in an improved OPS system with 50％ to 60％ of oocytes maturation. Cleavage was achieved following insemination after oocytes vitrification and maturation. However, further experiments are needed to improve the cleavage rate and further pregnancy following embryo transfer. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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