Ginkgo Biloba Extract Reduces Neointimal Hyperplasia and Interleukin-1 beta Expression after Balloon Injury in Cholesterol-Fed Rabbits
| Autor: | Der-Fang Yang, 楊德芳 |
|---|---|
| Rok vydání: | 1999 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 87 Neointimal hyperplasia, mainly the proliferation of smooth muscle cells and accumulation of macrophage-derived foam cells and interleukin-1 beta (IL-1 β) might play an important role in the progress, is one of major mechanisms responsible for postangioplasty restenosis. Ginkgo biloba extract (GBE) has been demonstrated to exhibit free-radical scavenging properties, antioxidant effects and platelet activating factor antagonism. The comparison of antioxidant capacity between GBE and probucol was determined by the inhibition of conjugated diene formation during peroxidation of human LDL by Cu2+ induction. The effects of GBE on cultured smooth muscle cell proliferation, neointimal hyperplasia and IL-1β expression after balloon injury of aorta in cholesterol-fed rabbits were studied. The antioxidant activity of GBE was 43% of that of probucol. GBE at concentrations of 50, 100, 200, 500, 1000 μg/ml were added to cultured smooth muscle cells and [3H]-thymidine uptake was determined. Male New Zealand white rabbits were fed with 2% cholesterol diet combined with daily oral ingestion of GBE (40mg/day; n=10), or probucol (250mg/day ; n=8), or none as control group (n=8) for 6 weeks. A balloon injury of abdominal aorta was performed at the end of 3rd week. The animals were sacrificed and abdominal aortas were harvested at the end of 6th week. Neointimal hyperplasia in abdominal aorta was determined morphometrically and the expression of IL-1β in arterial tissue was determined by Northern blot analysis. The results revealed that the antioxidant activity of GBE was 43% of that of probucol. GBE significantly inhibited proliferation of cultured smooth muscle cells at different concentrations and cholesterol-lowering effect. The atheroma area in thoracic aortas of GBE-treated group was less than that of control group (atheroma/total area ratio: 0.228±0.088 vs. 0.491±0.1048，p |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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