Establishing and Analysis of the Fingerprints of Ostriches
| Autor: | Yan-Chung Shih, 施彥仲 |
|---|---|
| Rok vydání: | 1999 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 87 The purpose of the studies was the establishing and analysis of RAPD and RAMPO fingerprints of ostriches to provide the genetic information and for the basic data of breeding strategy. The RAPD and RAMPO fingerprints of emus were also carried out and compared with the fingerprints of ostriches. The results showed that the average band sharing frequency of RAPD fingerprints amplified with 45 random primers in black neck ostriches within-population of different breeds was 80.4±7.1﹪(60.8-92.4﹪), 81.2±6.2﹪(65.3-90.3﹪)in blue neck birds and 89.0±7.2﹪(72.2-100.0﹪) in red neck birds, respectively. It revealed that the heterozygosity of genetics in black neck birds was higher than others. The band sharing frequency of RAPD of ostriches of inter-population and genetic distance was 96.3±7.6﹪ and 0.047 in black and blue neck birds, 86.4±11.1﹪ and 0.174 in black and red neck birds, 87.4±10.3﹪ and 0.159 in blue and red neck birds, respectively. It meant that the relationship between black and blue neck birds was more closely than others, and the results were according to the breeding course of black neck ostriches. The average band sharing frequency of RAPD fragments amplified with 45 random primers in black neck birds within-population distributed in 3 farms was estimated. It was 88.0±4.9﹪(75.0-97.0﹪) in G farm, 90.6±5.2﹪(76.3-97.6﹪) in T farm and 88.6±5.6﹪(70.4-97.9﹪) in L farm, respectively. The average band sharing frequency of T farm was higher than G farm significantly (P＜0.05). It meant that the heomozygosity of T farm was higher than G farm. The band sharing frequency of RAPD of inter-population and genetic distance in different farms of black neck birds were also evaluated. They were 90.7±7.2﹪ and 0.107 in G and T farms, 91.3±8.7﹪ and 0.104 in G and L farms, 92.3±7.1﹪ and 0.090 in T and L farms. The band sharing frequency of RAPD of inter-population of black neck birds in different farms was no significant difference (P＞0.05). The average band sharing frequency of RAPD fragments amplified with 45 random primers in blue neck birds within-population distributed in 2 farms was estimated. It was 90.0±4.5﹪(76.9-98.6﹪) in G farm, 91.9±4.8﹪(82.7-98.9﹪) in L farm, respectively. The average band sharing frequency of L farm was higher than G farm but no significant difference (P＞0.05). The band sharing frequency of RAPD of inter-population and genetic distance in different farms of blue neck birds was also evaluated. It was 91.5±8.7﹪ and 0.104 in G and T farms. In the species identification by using RAPD or RAMPO fingerprinting, many specific bands of RAPD fingerprints amplified with OPAO08, OPAO09, OPAA10 and OPAV09 primers each were observed in birds between ostriches and emus. The specific bands between them also found in the RAMPO fingerprints amplified with OPAO08 primer and hybridized with 32P-labeled (TG)6 probe. It showed that the RAPD or RAMPO techniques could be used for the identification between ostrich and emu meat products. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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