Studies on the anxiolytic effects of Suanzaoren in Rodents
| Autor: | Yi-Hsuan Lee, 李宜璇 |
|---|---|
| Rok vydání: | 1999 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 87 The present study was attempted to investigate the anxiolytic effects of the alcoholic or aquatic extracts of Ziziphus jujuba (abbreviated as SZA or SZW) by means of the black and the white test and elevated plus-maze. We further demonstrated the anxiolytic mechanism of total saponins of Ziziphus jujuba (SZTS) by combining with serotonergic agonists or antagonists, and measuring the concentrations of monoamines and their metabolites in the brain stem by high performance liquid chromatography plus electrochemical detector. SZA or SZW (0.5 and 1.0 g/kg) for single administration increased the time spent in the white compartment and total change between two compartments, and decreased the time spent in the black compartment of the black and white test. SZA or SZW (0.5 and 1.0 g/kg) for single administration increased the percentage of entries and percentage of the time spent on the open arms, and decreased the percentage of entries and percentage of the time spent on the close arms in the elevated plus-maze. On the other hand, SZA (1.0 and 2.0 g/kg) or SZW (0.5，1.0 and 2.0 g/kg) for single administration decreased the horizontal activity and prolonged the hexobarbital-induced sleeping times (sedative effect). Furthermore, SZTS (0.2，0.5 and 1.0 g/kg) for single administration had the anxiolytic effect in the black and white test and the elevated plus-maze in rodents, but did not have the sedative effect. SZTS at 1 g/kg decreased the concentrations of NE and DA, 5-HT, and increased the concentrations of 5-HIAA, VMA and HVA in the cotex and brain stem. SZTS also enhanced the anxiolytic effect of buspirone, p-MPPI and ritanserin, and attenuated the anxiolytic effect of DOI, 8-OH-DPAT and WAY-100635 in the black and white test. From these results, SZTS had the anxiolytic effect in the black and white test and the elevated plus-maze in rodents. The anxiolytic mechanisms of SZTS might be due to decreasing the concentration of 5-HT in brain stem by activating somatodendritic 5-HT1A receptors and inhibiting postsynaptic 5-HT1A and 5-HT2 receptors. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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