The genetic diversity of rice varieties and their ancetor revealed by DNA polymorphysom
| Autor: | Yang, Shou-Jean, 楊小珍 |
|---|---|
| Rok vydání: | 1998 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 86 Rice is a major crop in Taiwan and distributes in temperate, tropical and subtropical regions. Taiwanese rice varieties mainly consist of Japonica and Indica types. The genetic relationship among rice varieties and their ancestors was evaluated by pedigree analysis. And the study provides some information about genetic background of rice varieties. Therefore, the thesis objective was to investigate the genetic relationship among rice varieties and their ancestors at molecular level.The genetic diversity among 86 rice varieties (including 42 Japonica cultivars, 7 Indica cultivars, 31 ancestors, 4 foreign varieties, and 2 Tsai-yuan-chon from Japan) were evaluated by random amplified polymorphic DNA (RAPD). Twelve of 54 primers were sufficient to generate polymorphisms and produced 90 banding patterns. When all 12 primers were taken into consideration, 30.3% of banding patterns were polymorphic among Japonica varieties, and 43.59% among Indica varieties. The averaged genetic distance between Japonica varieties was 0.06, and 0.154 between Indica varieties. Therefore, the genetic diversity of Japonica cultivars is smaller than that of Indica cultivars. Clustering dendrograph based on 12 primers distinguishs Japonica cultivars from Indica cultivars. Taiwanese rice varieties generally showed closer affinity to their ancestors. For example, Iwata Asahi and Tainung 67 were clustered one group, because Iwata Asahi was one of ancestors of Tainung 67. The data based on cluster analysis was similar to those based on pedigree analysis. RAPD analysis was able to detect variants in landraces. For example, Tsai-yuan-chon from Taiwan Agricultural Research Institute was close affinity with Japonica cultivars, and two Tsai-yuan-chon from Japan showed close affinity to indica cultivars. Because of the lack of samples for Tsai-yuan-chon from different sources, it is necessary to increase the number of seed sources to recognize the genetic background of Tsai-yuan-chon. The results suggest the RAPD analysis in conjunction with pedigree information provide better estimates of genetic diversity than analysis those solely on pedigree information. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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