Studies on Saprolegniaceous Fungi in Taiwan
| Autor: | Fu, Chuen-Hsu, 傅春旭 |
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| Rok vydání: | 1997 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 85 More than 400 samples of soilsand water were collected from various locations of Taiwan. Baiting method was used to isolate the Saprolegniaceous fungi. Totally 28 species included in 8 genera of Saprolegniaceous fungi were identified. Among them, 3 species, ie. Achlya diffusa, A. inflata and Saprolegnia australis, are new records for Taiwan. The author screened 40 antibiotics and fungicides to make selective medium for isolation of Saprolegniaeous fungi. After screening work, the medium made of, adding 1 ppm Metalaxyl 、250 ppm PCNB、500 ppm Neomycin sulfate and 1000 ppm Amoxicillin to YpSs+hempseed extract medium shows the best selective effect of isolation of Saprolegniaeous fungi. In the survey on survival structures in the soil,oospores played the most important role in the survival of Saprolegniaeous fungi. In addition to its survivability,oospore was more resistant to the low temperature （-20℃）and chemicals （KMnO4）than cyst 、 hyphae and gemmae. In the germination experiment of Achlya prolifera, the oospores formed in gnobiotic culture gived higher germination ratio than that formed in axenic culture.Nutrients and high temperature could promote the germination of oospores. KMnO4 solutions and light showed no effect on promoting the germination of oospores. In addition to the resistant to the stress, the single spore isolates from oospores have more diversity in the growth raets than those single spore isolates from zoospores. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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