Rapid and Convenient Extraction of Ginsenosides from Panax Ginseng and Ginseng Drug Preparations Using Sep-pak C18 Cartridges
| Autor: | Tsai, Pai-Feng, 蔡百豐 |
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| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 74 Common preliminary procedures for the isolation of ginsenosides (triterpene saponins) from ginseng roots and leaves involve solvent extraction or extraction by adsorbents. Such methods have been time consuming and the use of multiple step procedures. In the present study, a simple and reproducible method was developed using a Sep-pak C18 cartridge as a clean-up step for the analysis of ginsenosides in ginseng roots, leaves and GINSANA -GI15 capsules. Relatively large volumes of water extract can be concentrated to 1.5 ml (in methanol) in a few minutes, and an aliquot of water-methanol extract by different cartridges results in ginsenoside profile analyses which are identical. The methanol extracts from the Sep-pak extraction were analyzed by high performance thin layer chromatography (HPTLC) and reverse phase high performance liquid chromatography (HPLC). Chromatography with HPTLC was done on Merck precoated kieselgel 60F254 plates (10 ×10 cm). As a developing solvent, CHCI3-MeOH-H2O (65:35:10, lower layer) mixture was used. The spots were detected by spraying 10% sulfuric acid followed by heating. For HPLC determinations, the separation is achieved under the following conditions: the mobile phase was 29% acetonitrile in water at a flow rate of 2 ml/min on □Bondapack C18 column or the mobile phase was 27% acetonitrile in water at a flow rate of 1.2 ml/min on TSKgel ODS-120T column. The ginsenosides are directly detected at 203 nm without derivatization. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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