| Popis: |
N.Crassa is known to utilize a salvage pathway for the production of uracil. This is known as the pyrimidine slavage pathway. This pathway consists of four enzymatic steps to convert thymidine to uracil. The final enzyme is isoorotate decarboxylase (IDCase), which forms uracil from isoorotate through a decarboxylation reaction. An in vitro assay for IDCase activity has been developed, which allowed the determination of specific activity of the enzyme in various strains of Neurospora. We attempted first to determine if another species of Neurospora contained this pathway by looking for the presence of the IDCase enzyme. |
