| Popis: |
In biopharmaceutical industry, protein-based drugs have been rapidly growing over the past decade. Approximately 40%-90% of total cost of protein drug manufacturing is spent on downstream processing. Due to protein target complexity, multiple chromatography purification steps are required to obtain products with high purity. Dr. Wood’s lab have been studying intein-mediated protein purification tag for years, one of the most significant discovery is the pH-sensitivity of intein cleaving reaction that result from zinc-binding motif. This work aims to investigate the mechanism of pH regulation on intein self-cleaving reaction and search for zinc-binding motif sequences with improved pH sensitivity. |
