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Staphylococcus pseudintermedius (SP) is the most common pathogen isolated from canine superficial pyoderma. Methicillin-resistant S. pseudintermedius (MRSP) is isolated with increasing frequency from these lesions.1 Doxycycline is the member of the tetracyclines that has been most commonly used to treat MRSP infections in dogs,2 while the use of minocycline has only been sporadically reported.3Clinical and Laboratory Standards Institute (CLSI) human tetracycline breakpoints to predict minocycline and doxycycline susceptibility of SP isolates from dogs are not appropriate because they do not meet pharmacokinetic/pharmacodynamic data using a standard dose. New breakpoints have been approved for doxycycline and proposed for minocycline and are four dilutions lower than tetracycline breakpoints, providing a more conservative standard for classification of isolates. The objectives of this study were to measure MICs of minocycline and doxycycline of 100 canine MRSP clinical isolates, compare their susceptibilities to minocycline and doxycycline based on current and revised standards, and document their tetracycline resistance genes. MICs were determined with E-test strips. PCR was used to identify tet genes. Using the human-derived tetracycline breakpoint of MIC |
