| Popis: |
A quantitative peroxidase-anti-peroxidase (PAP) method was developed and applied to the analysis of formalin-fixed Paraplast embedded tissue. The PAP method was used to ascertain the amounts of prostatic specific acid phosphatase (PSAP) in the tissue from patients with benign prostatic hypertrophy (BPH) and adenocarcinoma of the prostate (ADCP). In twenty-four cases of BPH, staining for PSAP was uniform and heavy. In twelve cases of ADCP, staining for PSAP was erratic and quantitatively lower than BPH tissues. Results from ADCP-PSAP tissue also indicated the presence or absence of PSAP was unrelated to the Gleason method of histological grading of ADCP. In addition the use of tissue localization of PSAP in the evaluation of primary ADCP and its metastases is discussed. |
