EFFECTS OF HUMAN HAIR KERATIN ALKYLATION ON GROWTH FACTOR DELIVERY AND CELLULAR RESPONSE: IMPLICATIONS IN BONE REGENERATION
| Autor: | Han, Sangheon |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: | |
| Druh dokumentu: | Text |
| Popis: | Bone injury due to congenital defects or injury is a clinical challenge. Keratins are structural intermediate proteins that can be obtained from multiple sources including human hair. Keratins are characterized by a high cysteine residue content in their primary structure, leading to the ability to form covalent disulfide crosslinks. This inherent cysteine content provides a chemical mechanism by which to control the degradation rate of keratin hydrogels: modulation of disulfide crosslinking. This thesis work seeks to understand cellular and bone morphogenetic protein 2 (BMP-2) interaction with keratins modified with alkyl groups at cysteine residues to achieve different degrees of disulfide crosslinking. Insulin-like growth factor 1 (IGF-1) and ciprofloxacin were also examined to better understand the delivery mechanism of drugs from these alkylated keratin biomaterials. The results of this thesis provide a better understanding of the mechanisms by which keratin hydrogels may serve as a platform for treatment of bone injuries. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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