ACF7 DEFICIENCY DOES NOT IMPAIR AUDITORY HAIR CELL DEVELOPMENT OR HEARING FUNCTION
| Autor: | Gilbert, Benjamin Lawrence |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Biology
Biomedical Research Molecular Biology Physiology Genetics Audiology hair cell inner hair cell outer hair cell hearing auditory system organ of corti cuticular plate cilia ACF7 Macf1 conditional knockout actin binding proteins microtubule binding proteins molecular biology cell biology developmental biology |
| Druh dokumentu: | Text |
| Popis: | ACF7 (actin crosslinking family protein-7) is a large actin-bundling and microtubule/actin crosslinking protein that is primarily responsible for cytoskeletal organization and integrity. Hair cells contain specialized subcellular structures to enable hearing, including the actin-rich cuticular plate and the circumferential band. ACF7 localizes to the cuticular plate and circumferential band of both mice and zebrafish. Due to known roles of ACF7 in the cytoskeleton of cell types throughout metazoans, it is plausible that ACF7 plays a vital role in the subcellular architecture of hair cell. To determine the function of ACF7 in hair cells, we used a Pax2-Cre/loxP system that removes three exons of the Macf1 actin binding domain in all descending cells of Pax2-expressing otic progenitors. Surprisingly, our data in four-week old conditional knockout mice suggests that hair cells have normal survivability, morphology, polarity, and hearing capabilities, demonstrating that the loss of ACF7 does not impact hearing in adult mice. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|