Improving Skin Wound Healing Using Functional Electrospun Wound Dressings and 3D Printed Tissue Engineering Constructs
| Autor: | Nun, Nicholas |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Animals
Biochemistry Biomedical Engineering Biomedical Research Chemistry Experiments Histology Materials Science Microbiology Molecules Organic Chemistry Plastics Polymer Chemistry Polymers wound healing electrospinning wound dressing 3d printing tissue engineering antimicrobial peptide conjugation quantitative histology polyester in vivo animal model infection model bioplotting acute wound rat model infected wound mouse model |
| Druh dokumentu: | Text |
| Popis: | Wound dressings play a pivotal role in providing favorable wound healing outcomes. Active dressings which incorporate bioactive agents such as anti-inflammatory drugs, antimicrobial compounds or peptides and proteins can help to alleviate patient pain and reduce healing times. This is especially true for diabetic ulcers, which are wounds that are stuck in the inflammatory stage of wound healing and are often associated with bacterial infection. This work describes the development of wound dressings capable of improving healing outcomes. To do so, a series of functional polyesters derived from a modular N-substituted diol monomer platform were synthesized. Wound dressings comprising these polyesters were fabricated using the 3D printing and electrospinning techniques. Dyes serving as model drug compounds were released from electrospun dressings to show the ability of these dressings to deliver drugs. The two fabrication techniques were used to create dressings comprised of varying polyester compositions. An in vivo acute wound rat model showed that despite their varying compositions and thread sizes, none of them were detrimental to normal healing processes. Electrospun dressings showed broad spectrum antimicrobial activity in an acute infected wound mouse model when an antimicrobial poly(ester urethane) was incorporated. Finally, 3D printed scaffolds were conjugated with peptides capable of recruiting keratinocytes and fibroblasts in an attempt to improve healing outcomes in an acute wound rat model. These studies seek to guide future researchers in the design and implementation of active wound dressings, especially by showing the utility of functionality within those dressing systems. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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