Transgenic assays for the analysis of DNA repair in plants
| Autor: | Ilnytskyy, Yaroslav, University of Lethbridge. Faculty of Arts and Science |
|---|---|
| Rok vydání: | 2005 |
| Předmět: | |
| Druh dokumentu: | Diplomová práce |
| Popis: | In this work we studied various aspects of DNA repair in plants, focusing mainly on point mutation repair and its interconnection with double-strand break repair. We were using transgenic point mutation and recombination substrates as a primary tool in our experiments. We have compared two transgenic homologous recombination assays (B-glucuronidase- and luciferase-based), analyzed the sensitivity of DNA repair machinery to ultraviolet radiation and assessed the involvement of AtKu80, Atm and AtXpd repair genes in point mutation repair. Ours study revealed the following: the luciferase-based recombination assay is more sensitive then B-glucuronidase-based; double-stand break repair machinery is sensitive to ultraviolet radiation, which results in increased pint mutation formation; chosen DNA repair genes might be impaired in point mutation repair, however further experimentations are needed to confirm this. xi, 132 leaves : ill. ; 29 cm. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|