Autor: |
Brailoiu, G. Cristina, Dun, Siok L., Chi, Michelle, Ohsawa, Masahiro, Chang, Jaw Kang, Yang, Jun, Dun, Nae J. |
Předmět: |
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Zdroj: |
ETSU Faculty Works. |
Druh dokumentu: |
Text |
DOI: |
10.1016/S0006-8993(03)03161-5 |
Popis: |
Beacon is a 73-amino acid peptide encoded by a novel gene in the hypothalamus of Israeli sand rat Psammomys obesus. Reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemical techniques were used to investigate the presence of beacon mRNA and the distribution of beacon-immunoreactivity (irBC) in the hypothalamus of ICR mice. RT-PCR experiments revealed beacon mRNA in the mouse hypothalamus. Using a rabbit polyclonal antiserum directed against the synthetic C-terminal peptide fragment (47-73), irBC was detected in the mouse hypothalamus and pituitary. In the hypothalamus, irBC was concentrated in perikarya of the supraoptic (SO), paraventricular (PVH) and accessory neurosecretory nuclei and in cell processes of the median eminence and pituitary stalk. In the pituitary, irBC was noted mainly in the posterior lobe. Double-labeling the hypothalamic sections with guinea-pig vasopressin-antiserum or mouse monoclonal oxytocin-antibody and beacon-antiserum revealed that |
Databáze: |
Networked Digital Library of Theses & Dissertations |
Externí odkaz: |
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