| Autor: |
Krieghoff, Jan, Rost, Johannes, Kohn-Polster, Caroline, Müller, Benno M., Koenig, Andreas, Flath, Tobias, Schulz-Siegmund, Michaela, Schulze, Fritz-Peter, Hacker, Michael C. |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
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| Druh dokumentu: |
Článek |
| ISSN: |
2227-9059 |
| Popis: |
The performance of artificial nerve guidance conduits (NGC) in peripheral nerve regeneration can be improved by providing structures with multiple small channels instead of a single wide lumen. 3D-printing is a strategy to access such multi-channeled structures in a defined and reproducible way. This study explores extrusion-based 3D-printing of two-component hydrogels from a single cartridge printhead into multi-channeled structures under aseptic conditions. The gels are based on a platform of synthetic, anhydride-containing oligomers for cross-linking of gelatinous peptides. Stable constructs with continuous small channels and a variety of footprints and sizes were successfully generated from formulations containing either an organic or inorganic gelation base. The adjustability of the system was investigated by varying the cross-linking oligomer and substituting the gelation bases controlling the cross-linking kinetics. Formulations with organic N-methyl-piperidin-3-ol and inorganic K2HPO4 yielded hydrogels with comparable properties after manual processing and extrusion-based 3D-printing. The slower reaction kinetics of formulations with K2HPO4 can be beneficial for extending the time frame for printing. The two-component hydrogels displayed both slow hydrolytic and activity-dependent enzymatic degradability. Together with satisfying in vitro cell proliferation data, these results indicate the suitability of our cross-linked hydrogels as multi-channeled NGC for enhanced peripheral nerve regeneration. |
| Databáze: |
Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
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