| Popis: |
The role of conventional TCRab+CD4+ or TCRab+CD8a+ single-positive (sp) T lymphocytes in adaptive immunity is well-recognized. However, non-conventional T cells expressing TCRab or TCRgd but lacking CD4 and CD8a expression [i.e., CD4−CD8a− double-negative (dn) T cells] are thought to play a role at the interface between the innate and adaptive immune system. Dn T cells are frequent in swine, cattle or sheep and predominantly express TCRgd. In contrast, TCRgd+ T cells are rare in dogs. In this study, we identified a high proportion of canine dn T cells in the TCRab+ T cell population of PBMC, lymphatic and non-lymphatic organs. In PBMC, the frequency of this T cell subpopulation made up one third of the frequency of TCRab+CD4+ sp, and almost half of the frequency of TCRab+CD8a+ sp T cells (i.e., ∼15% of all TCRab+ T cells). Among TCRab+CD4−CD8a− dn T cells of PBMC and tissues, FoxP3+ cells were identified indicating regulatory potential of this T cell subset. 80% of peripheral blood FoxP3+TCRab+CD4−CD8a− dn T cells co-expressed CD25, and, interestingly, also the FoxP3-negative TCRab+CD4−CD8a− dn T cells comprised ∼34% CD25+ cells. Some of the FoxP3-positive TCRab+CD4−CD8a− dn T cells co-expressed GATA-3 suggesting stable function of regulatory T cells. The frequency of GATA-3 expression by FoxP3−TCRab+CD4−CD8a− dn T cells was even higher as compared with TCRab+CD4+ sp T cells (20.6% vs. 11.9%). Albeit lacking FoxP3 and CD25 expression, TCRgd+CD4−CD8a− dn T cells also expressed substantial proportions of GATA-3. In addition, TCRab+CD4−CD8a− dn T cells produced IFN-g and IL-17A upon stimulation. T-bet and granzyme B were only weakly expressed by both dn T cell subsets. In conclusion, this study identifies two dn T cell subsets in the dog: (i) a large (∼7.5% in Peyer’s patches, ∼15% in lung) population of TCRab+CD4−CD8a− dn T cells with subpopulations thereof showing an activated phenotype, high expression of FoxP3 or GATA-3 as well as production of IFN-g or IL-17A and (ii) a small TCRgd+CD4−CD8a− dn T cell subset also expressing GATA-3 without production of IFN-g or IL-17A. It will be exciting to unravel the function of each subset during immune homeostasis and diseases of dogs. |
