A single-cell view on the intra- and inter-population metabolic heterogeneity and ecophysiology of microorganisms at different ecological scales
| Autor: | Calabrese, Federica |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
metabolic heterogeneity
single-cell analysis stable isotope probing combined with nanoSIMS heterogeneity quantitation assimilation rates Stoffwechselheterogenität Einzelzellanalyse stabile Isotope nanoSIMS Quantifizierung der Heterogenität Assimilationsraten info:eu-repo/classification/ddc/570 ddc:570 Mikroorganismus Stoffwechsel Einzelzellanalyse Sekundärionen-Massenspektrometrie Stabiles Isotop Heterogenität |
| Druh dokumentu: | Text<br />Doctoral Thesis |
| DOI: | 10.1111/1462-2920.15756 |
| Popis: | Metabolic heterogeneity (MH) occurs when isogenic microbial populations display cell-to-cell differences in metabolic traits, albeit exposed to homogeneous conditions. Despite the increasing focus on MH, its triggering factors remain largely unknown. In the present thesis, I used stable isotope probing and chemical imaging with nanoscale Secondary Ion Mass Spectrometry (nanoSIMS) to study MH at single-cell level, in model organisms, synthetic and natural communities, to understand i) how abiotic factors, biotic interactions and antibiotics exposure influence MH and ii) its potential ecological role. Moreover, I optimized sample preparation for chemical and high-resolution imaging and suggested two different indices as ‘unit measure’ of MH. As results, I have shown for the first time that MH is displayed by microorganisms under favorable growth conditions, although none of the tested abiotic factors prevailed as the main trigger of MH. I brought insights on how biotic interactions play a role in the functional heterogeneity using bacteria pseudo-fungi co-cultures. I found that antibiotics reduce Carbon and Nitrogen assimilation rates of targeted phylogenetic groups in river-water communities, while increasing their MH, pointing to its ecological importance in natural environments. To conclude, I provided novel insights on the phenomenon of MH and its dynamics at different ecological scales.:Abbreviation list Summary Introduction Knowledge gaps Results and discussion - Optimization of sample preparation - Validation of quantitation methods - Abiotic factors shaping metabolic heterogeneity in bacterial populations - Influence of biotic factors in shaping heterogeneity - Metabolic Heterogeneity and ecophysiology of natural microbial populations influenced by emerging contaminants Conclusions Outlook Bibliography Appendix Acknowledgments Curriculum Vitae List of publications |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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