Mapping of conserved RNA secondary structures predicts thousands of functional noncoding RNAs in the human genome
| Autor: | Washietl, Stefan, Hofacker, Ivo L., Lukasser, Melanie, Hüttenhofer, Alexander, Stadler, Peter F. |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: | |
| Druh dokumentu: | Článek |
| ISSN: | 1087-0156 1546-1696 |
| Popis: | In contrast to the fairly reliable and complete annotation of the protein coding genes in the human genome, comparable information is lacking for noncoding RNAs (ncRNAs). We present a comparative screen of vertebrate genomes for structural noncoding RNAs, which evaluates conserved genomic DNA sequences for signatures of structural conservation of base-pairing patterns and exceptional thermodynamic stability. We predict more than 30,000 structured RNA elements in the human genome, almost 1,000 of which are conserved across all vertebrates. Roughly a third are found in introns of known genes, a sixth are potential regulatory elements in untranslated regions of protein-coding mRNAs and about half are located far away from any known gene. Only a small fraction of these sequences has been described previously. A comparison with recent tiling array data shows that more than 40% of the predicted structured RNAs overlap with experimentally detected sites of transcription. The widespread conservation of secondary structure points to a large number of functional ncRNAs and cis-acting mRNA structures in the human genome. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|