| Autor: |
Ottenheim, Christoph, Verdejo, Carl, Zimmermann, Wolfgang, Wu, Jin Chuan |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
|
| Druh dokumentu: |
Článek |
| DOI: |
10.1016/j.jbiosc.2014.05.014 |
| Popis: |
Palm oil empty fruit bunches (EFB) is an abundant and cheap lignocellulose material in South East Asia. Its use as the sole medium for producing lignocellulose-hydrolyzing enzymes would increase its commercial value. A newly isolated Aspergillus niger DSM 26641 was investigated for its capability of producing hemicellulases in EFB hydrolysate obtained by treatment with pressurized hot water (1-20%, w/v) at 120-180◦C in a 1 L Parr reactor for 10-60 min. The optimal hydrolysate for the fungal growth and endoxylanase production was obtained when 10% (w/v) of empty fruit bunch was treated at 120◦C or 150◦C for 10 min, giving an endoxylanase activity of 24.5 mU ml-1 on RBB-Xylan and a saccharification activity of 5 U ml-1 on xylan (DNS assay). When the hydrolysates were produced at higher temperatures, longer treatment times or higher biomass contents, only less than 20% of the above maximal endoxylanase activity was detected, possibly due to the higher carbohydrate concentrations in the medium. Transcriptome analysis showed that 3 endoxylanases (expression levels 59-100%, the highest level was set as 100%), 2 b-xylosidases (4%), 4 side chain-cleaving arabinofuranosidases (1-95%), 1 acetyl xylan esterase (9%) and 2 ferulic acid esterases (0.3-9%) were produced together. |
| Databáze: |
Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|
