Rational development of novel activity probes for the analysis of human cytochromes P450

Autor:	Sellars, J.D., Skipsey, M., Sadr-ul-Shaheed, Gravell, Sebastian, Abumansour, Hamza M.A., Kashtl, Ghasaq J., Irfan, Jawaria, Khot, Mohamed, Pors, Klaus, Patterson, Laurence H., Sutton, Chris W.
Jazyk:	angličtina
Rok vydání:	2016
Předmět:	Human cytochromes P450 Functional cytochromes CYPs Drug efficacy Probe binding NADPH
Druh dokumentu:	Článek
DOI:	10.1002/cmdc.201600134
Popis:	Yes The identification and quantification of functional cytochromes P450 (CYPs) in biological samples is proving important for robust analyses of drug efficacy and metabolic disposition. In this study, a novel CYP activity-based probe was rationally designed and synthesised, demonstrating selective binding of CYP isoforms. The dependence of probe binding upon the presence of NADPH permits the selective detection of functionally active CYP. This allows the detection and analysis of these enzymes using biochemical and proteomic methodologies and approaches. Engineering and Physical Sciences Research Council (EPSRC) and Yorkshire Cancer Research
Databáze:	Networked Digital Library of Theses & Dissertations
Externí odkaz:	http://hdl.handle.net/10454/8900 Zobrazit plný text záznamu