| Popis: |
Hybridoma cells have been used as targets to measure cytotoxic cells in a technique which uses the secretion of monoclonal antibody as an index of viability and a means of measuring the number of cells by the haemolytic plaque technique. The hybridoma has been shown to be susceptible to cytotoxic T cells, natural killer cells and cytotoxic cells arising spontaneously in culture. From the original cloned hybridoma line, a number of variants have been isolated which grow as different types of tumours in syngeneic mice. One particular strain grows with high efficiency as tumours in the spleen. In contrast to the original hybridoma, the progressive spleen-seeking tumour cells appear to have lost their ability to stimulate the immune system. The contribution that natural killer cell and T cell immunity plays in controlling tumour growth has been demonstrated and the vitro analysis of immune effector cells in the spleen of mice challanged with tumour cells has been investigated. These data illustrate the advantages of using hybridoma cells as a model to investigate tumour immunity. |
