| Autor: |
André Olean-Oliveira, Patricia Monteiro Seraphim, Marcos F.S. Teixeira |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Electrochemistry Communications, Vol 136, Iss , Pp 107242- (2022) |
| Druh dokumentu: |
article |
| ISSN: |
1388-2481 |
| DOI: |
10.1016/j.elecom.2022.107242 |
| Popis: |
The present work describes the development of an electrochemical immunosensor for the direct determination of methylated DNA using dissolved oxygen as the redox probe. The oxygen-sensitive response is possible due to the presence of a redox conducting polymer based on the Bismarck Brown Y dye (poly(azo-BBY)). In addition to the azo-polymer, gold nanoparticles (AuNPs) were formed in the polymer film by encapsulation to increase the active surface area and enhance sensor performance. The platform was easily synthesized using a single-step electropolymerization technique in a solution containing the BBY monomer and HAuCl4 salt. The immunosensor was developed by simply immobilizing the 5-methylcytosine antibody (Ab-5-mC) with high affinity to 5-mC on the surface of a device coated with azo-polymer-AuNPs dots. No secondary antibodies or enzymes were used in constructing this device. The immunosensor performance was evaluated by electrochemical impedance spectroscopy (EIS) in different concentrations of 5-methylcytosine (5-mC) in 0.10 mol L−1 phosphate buffer solution (PBS) solution containing atmospheric oxygen. A calibration curve was obtained by varying the resistance of the system as a function of the 5-mC concentration, revealing a limit of detection (LOD) in picograms per milliliter. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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Full Text from ScienceDirect