Chromosome-level genome assembly of Acrossocheilus fasciatus using PacBio sequencing and Hi-C technology

Autor: Jianbo Zheng, Jianhu Jiang, Qianlong Rui, Fei Li, Shili Liu, Shun Cheng, Meili Chi, Wenping Jiang
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Scientific Data, Vol 11, Iss 1, Pp 1-9 (2024)
Druh dokumentu: article
ISSN: 2052-4463
DOI: 10.1038/s41597-024-02999-6
Popis: Abstract Acrossocheilus fasciatus (Cypriniformes, Cyprinidae) is emerged as a newly commercial stream fish in the south of China with high economic and ornamental value. In this study, a chromosome-level reference genome of A. fasciatus was assembled using PacBio, Illumina and Hi-C sequencing technologies. As a result, a high-quality genome was generated with a size of 879.52 Mb (accession number: JAVLVS000000000), scaffold N50 of 32.7 Mb, and contig N50 of 32.7 Mb. The largest and smallest scafford was 60.57 Mb and 16 kb, respectively. BUSCO analysis showed a completeness score of 98.3%. Meanwhile, the assembled sequences were anchored to 25 pseudo-chromosomes with an integration efficiency of 96.95%. Additionally, we found approximately 390.91 Mb of repetitive sequences that accounting for 44.45% of the assembled genome, and predicted 24,900 protein-coding genes. The available genome reported in the present study provided a crucial resource to further investigate the regulation mechanism of genetic diversity, sexual dimorphism and evolutionary histories.
Databáze: Directory of Open Access Journals