| Autor: |
Saptaparni Bandyopadhyay, Joseph Douglass, Sebastian Kapell, Nazimuddin Khan, Fabiana Feitosa-Suntheimer, Jenny A Klein, Jasmine Temple, Jayce Brown-Culbertson, Alexander H Tavares, Mohsan Saeed, Nelson C Lau |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
G3: Genes, Genomes, Genetics, Vol 11, Iss 8 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2160-1836 |
| DOI: |
10.1093/g3journal/jkab169 |
| Popis: |
AbstractKnock-in of large transgenes by Cas9-mediated homology-directed repair (HDR) is an extremely inefficient process. Although the use of single-stranded oligonucleotides (ssODN) as an HDR donor has improved the integration of smaller transgenes, they do not support efficient insertion of large DNA sequences. In an effort to gain insights into the mechanism(s) governing the HDR-mediated integration of larger transgenes and to improve the technology, we conducted knock-in experiments targeting the human EMX1EMX1ViperinRSAD2ISG15 |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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