Co-supplementation of freezing media with trehalose and vitamin C on cell viability and apoptotic gene expression in ovine spermatogonial stem cells
| Autor: | R. Asadpour, S. Kalantari, A. A. Shahbazfar, R. Jafari-Jozani |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Bulgarian Journal of Veterinary Medicine, Vol 25, Iss 4, Pp 593-602 (2022) |
| Druh dokumentu: | article |
| ISSN: | 1311-1477 1313-3543 |
| DOI: | 10.15547/bjvm.2020-0068 |
| Popis: | The purpose of this research was to investigate the trehalose and vitamin C (Vit C) co-supplementation of freezing media to create a successful cryopreservation protocol for conservation of ovine spermatogonial stem cells (SSCs). SSCs were cryopreserved and cultured with an essential freezing medium containing 200 mM trehalose, 40 µg/mL Vit C, and a combination of both for 3 weeks. Cell viability, colony number and diameter and mRNA levels of Bax, and Bcl-2 genes were evaluated before and after cryopreservation with quantitative real-time PCR. The results showed that cells cryopreserved in freezing medium containing 200 mM of trehalose plus 40 µg/mL Vit C had considerably greater cell viability than the control group (P |
| Databáze: | Directory of Open Access Journals |
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