Antimicrobial potential of AH Plus supplemented with bismuth lipophilic nanoparticles on isolated from clinical isolates

Autor: Jesús Alejandro Torres-Betancourt, Rene Hernandez-Delgadillo, Jorge Jaime Flores-Treviño, Juan Manuel Solís-Soto, Nayely Pineda-Aguilar, Maria Argelia Akemi Nakagoshi-Cepeda, Rosa Isela Sánchez-Nájera, Shankararaman Chellam, Claudio Cabral-Romero
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Journal of Applied Biomaterials & Functional Materials, Vol 20 (2022)
Druh dokumentu: article
ISSN: 2280-8000
22808000
DOI: 10.1177/22808000211069221
Popis: The objective of this study was to determine the antimicrobial potential of AH plus supplemented with bismuth lipophilic nanoparticles (BisBAL NPs) on the growth of Enterococcus faecalis isolated from patients with endodontic infections. BisBAL NPs, synthesized with the colloidal method, were characterized, in its pure form or AH Plus-absorbed, by energy-dispersive X-ray spectroscopy and scanning electron microscopy (EDS-SEM). Antimicrobial activity was evaluated with disc diffusion assays, and antibiofilm activity with fluorescence microscopy. BisBAL NP-supplemented AH Plus had a 4.9 times higher antimicrobial activity than AH Plus alone ( p = 0.0001). In contrast to AH Plus alone, AH Plus supplemented with BisBAL NP inhibited E. faecalis biofilm formation. The sealing properties of AH plus were not modified by the incorporation of BisBAL NPs, which was demonstrated by a 12-day split-chamber leakage assay with daily inoculation, which was used to evaluate the possible filtration of E. faecalis . Finally, BisBAL NP-supplemented AH plus-BisBAL NPs was not cytotoxic for cultured human gingival fibroblasts. Their viability was 83.7% to 89.9% after a 24-h exposure to AH Plus containing 50 and 10 µM BisBAL NP, respectively. In conclusion, BisBAL NP-supplemented AH Plus constitutes an innovative nanomaterial to prevent re-infection in endodontic patients without cytotoxic effects.
Databáze: Directory of Open Access Journals
Externí odkaz: