Ultrasensitive anti-melamine monoclonal antibody and its use in the development of an immunochromatographic strip
| Autor: | Mengyuan Guo, Xiaoling Wu, Shanshan Song, Qiankun Zheng, Pengjie Luo, Hua Kuang, Jiajia Sun, Liya Ye |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Food and Agricultural Immunology, Vol 30, Iss 1, Pp 462-474 (2019) |
| Druh dokumentu: | article |
| ISSN: | 0954-0105 1465-3443 09540105 |
| DOI: | 10.1080/09540105.2019.1590318 |
| Popis: | The hapten 1 which was formed by the reaction of 2-chloro-4,6-diamine-1,3,5-triazine (CAAT) with 6-aminocaproic acid, conjugated with keyhole limpet haemocyanin to prepare the immunogen. And coating antigen was prepared by the coupling of ovalbumin and hapten 2 which was synthesized by the reaction of CAAT and 3-mercapto propionic acid. Based on immunological and cell fusion technology, the anti-melamine monoclonal antibody was prepared and characterized. The half inhibitory concentration of the antibody for melamine was 1.8 ng/mL, and the linear range was 0.61–5.56 ng/mL. An immunochromatographic (ICT) strip, based on the antibody, was developed for the rapid detection of melamine residues in milk samples. The ICT strip had a visual cut-off value of 10 ng/mL for milk samples. Therefore, the colloidal gold immunoassay with the advantage of high affinity and sensitivity can be used for the ultrasensitive detection of melamine residues in milk samples. |
| Databáze: | Directory of Open Access Journals |
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