| Autor: |
Rajagopala Seesandra V, Yamamoto Natsuko, Zweifel Adrienne E, Nakamichi Tomoko, Huang Hsi-Kuang, Mendez-Rios Jorge, Franca-Koh Jonathan, Boorgula Meher, Fujita Kazutoshi, Suzuki Ken-ichirou, Hu James C, Wanner Barry L, Mori Hirotada, Uetz Peter |
| Jazyk: |
angličtina |
| Rok vydání: |
2010 |
| Předmět: |
|
| Zdroj: |
BMC Genomics, Vol 11, Iss 1, p 470 (2010) |
| Druh dokumentu: |
article |
| ISSN: |
1471-2164 |
| DOI: |
10.1186/1471-2164-11-470 |
| Popis: |
Abstract Background Systems biology and functional genomics require genome-wide datasets and resources. Complete sets of cloned open reading frames (ORFs) have been made for about a dozen bacterial species and allow researchers to express and study complete proteomes in a high-throughput fashion. Results We have constructed an open reading frame (ORFeome) collection of 3974 or 94% of the known Escherichia coli K-12 ORFs in Gateway® entry vector pENTR/Zeo. The collection has been used for protein expression and protein interaction studies. For example, we have compared interactions among YgjD, YjeE and YeaZ proteins in E. coli, Streptococcus pneumoniae, and Staphylococcus aureus. We also compare this ORFeome with other Gateway-compatible bacterial ORFeomes and show its utility for comparative functional genomics. Conclusions The E. coli ORFeome provides a useful resource for functional genomics and other areas of protein research in a highly flexible format. Our comparison with other ORFeomes makes comparative analyses straighforward and facilitates direct comparisons of many proteins across many genomes. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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