Autor: |
Durigon Edison L, do SM Castro Jesuína, de Araujo Renato F, de S Orrico Agnaldo, de S Leandro André, Amarilla Alberto A, de C Gomes Almério, de Figueiredo Mario LG, Aquino Victor H, Figueiredo Luiz TM |
Jazyk: |
angličtina |
Rok vydání: |
2010 |
Předmět: |
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Zdroj: |
Virology Journal, Vol 7, Iss 1, p 152 (2010) |
Druh dokumentu: |
article |
ISSN: |
1743-422X |
DOI: |
10.1186/1743-422X-7-152 |
Popis: |
Abstract Dengue epidemics have been reported in Brazil since 1985. The scenery has worsened in the last decade because several serotypes are circulating and producing a hyper-endemic situation, with an increase of DHF/DSS cases as well as the number of fatalities. Herein, we report dengue virus surveillance in mosquitoes using a Flavivirus genus-specific RT-Hemi-Nested-PCR assay. The mosquitoes (Culicidae, n = 1700) collected in the Northeast, Southeast and South of Brazil, between 1999 and 2005, were grouped into 154 pools. Putative genomes of DENV-1, -2 and -3 were detected in 6 mosquito pools (3.8%). One amplicon of putative DENV-1 was detected in a pool of Haemagogus leucocelaenus suggesting that this virus could be involved in a sylvatic cycle. DENV-3 was found infecting 3 pools of larvae of Aedes albopictus and the nucleotide sequence of one of these viruses was identified as DENV-3 of genotype III, phylogenetically related to other DENV-3 isolated in Brazil. This is the first report of a nucleotide sequence of DENV-3 from larvae of Aedes albopictus. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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