Construction of a Peptide Microarray for Auto-anti- body Detection
| Autor: | Vincent Cosandey, Fabien Debrot, Jérémy Kaeser, Roger Marti, Philippe Passeraub, Jannick Pétremand, Denis Prim, Marc E. Pfeifer |
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| Jazyk: | German<br />English<br />French |
| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | CHIMIA, Vol 66, Iss 10 (2012) |
| Druh dokumentu: | article |
| ISSN: | 0009-4293 2673-2424 |
| DOI: | 10.2533/chimia.2012.803 |
| Popis: | Peptide and protein microarrays provide a multiplex approach to identification and quantification of protein–protein interactions (PPI), useful to study for instance antigen–antibody properties. Multivariate serology assays detecting multiple tumor auto-antibodies (TAA) is an emerging class of blood tests for cancer detection. Here we describe the efficient coupling of peptide baits derived from the BRCA1-associated RING domain protein 1 (BARD1) to a solid surface and detection of a commercially available anti-BARD1 antibody with this newly designed peptide microarray. Analytical sensitivity and specificity were shown to be comparable to a microtiter plate based enzyme-linked immunosorbent assay (ELISA). |
| Databáze: | Directory of Open Access Journals |
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