تحدید الجین vanA المشفر لمقاومة الفانکومایسین على الدنا الکروموسومی والبلازمیدات فی المکورات العنقودیة الذهبیة المعزولة من خمج الجروج.
| Autor: | Mushtaq T. Salih, Ahmed M. Turkey, Mayada A. Shihan |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | مجلة جامعة الانبار للعلوم الصرفة, Vol 9, Iss 3, Pp 166-171 (2015) |
| Druh dokumentu: | article |
| ISSN: | 1991-8941 2706-6703 |
| DOI: | 10.37652/juaps.2015.127640 |
| Popis: | Staphylococcus aureus is one of the common causes of hospital and community acquired infections and treatment of Staphylococcal infections is complication because ability of this bacteria species to resistant to antibiotics .In this study vancomycin resistant Staphylococcus aureus were grew on Muller hintone agar contain vancomycin (6µg / ml) with percentage 25% (24/96) , Genomic DNA and plasmids isolated from isolates using standard producers. VanA gene amplification using PCR and suitable primers. The amplification gene was produced 1032bp at agarose gel electrophoresis .VanA gene was detected in 50% (12/24) on chromosomal DNA and in 20.8% (5/25) on plasmid of vancomycin resistant Staphylococcus aureus. |
| Databáze: | Directory of Open Access Journals |
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