The genetic variation of different developmental stages of Schistosoma japonicum: do the distribution in snails and pairing preference benefit the transmission?

Autor: Meng-Jie Gu, Yan-Wei Li, Aidan M. Emery, Shi-Zhu Li, Yong-Zhong Jiang, Hui-Fen Dong, Qin-Ping Zhao
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Parasites & Vectors, Vol 13, Iss 1, Pp 1-13 (2020)
Druh dokumentu: article
ISSN: 1756-3305
DOI: 10.1186/s13071-020-04240-w
Popis: Abstract Background Schistosoma japonicum is a waterborne parasite that causes schistosomiasis in humans and in more than 40 animal species. Schistosoma japonicum shows distinct genetic differentiation among geographical populations and multiple hosts, but the genetic diversity of different developmental stages of S. japonicum from is less studied. Such studies could elucidate ecological mechanisms in disease transmission by analysing feedbacks in individual physiology and population state. Methods After infection using cercariae from a pool of snails shedding together (Method I) and infection using mixed equal numbers of cercariae from individually shed snails (Method II), different developmental stages of S. japonicum were genotyped with microsatellite loci, including 346 cercariae, 701 adult worms and 393 miracidia. Genetic diversity and molecular variation were calculated at different population levels. Kinships (I′) among cercariae at intra-snail and inter-snail levels were evaluated. Genetic distance (Dsw) was compared between paired and unpaired worms, and partner changing was investigated through paternity identification for miracidia. Results The cercaria clones in individual snails varied from 1 to 8 and the kinship of cercariae within individual snails was significant higher (P
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