| Autor: |
Ivan Mikšík, Šárka Kubinová, Marine Morvan, Karel Výborný, Ameneh Tatar, Vladimír Král, Kamil Záruba, David Sýkora |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Separations, Vol 7, Iss 3, p 49 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2297-8739 |
| DOI: |
10.3390/separations7030049 |
| Popis: |
Chondroitin sulphates belong to a group of naturally occurring glycosaminoglycans and play a role in many physiological processes including ageing and the effects of various diseases. Research into chondroitin sulphates has found that the most important analytes are 4- and 6-sulphated disaccharides. We developed an HPLC method for the separation and quantification of underivatized chondroitin/dermatan sulphates—unsaturated disaccharides (4- and 6-sulphated disaccharides). This method is based on the separation of disaccharides by amido as well as amino columns under acidic conditions. These columns enabled the successful separation of 4- and 6-sulphated disaccharides using 50 (amido column) and 25 mmol/L (amino column) phosphate buffer, pH 4.25 (detection at 230 nm), at retention times of less than 10 min. The limit of quantification was 0.5 μg/mL. The applicability of this method was demonstrated through analysis of unsaturated disaccharides produced from the enzymatic digestion of chondroitin/dermatan sulphates of the solubilized extracellular matrix produced from porcine urinary bladder and human umbilical cord. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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